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(a) Transcriptomic analysis of the time-course differentiation process. Loss of pluripotency markers is visible over time. (b) A GM25256 hiPSC colony after 12 h of RA-induced differentiation. The red arrow indicates filopodium-like membrane protrusion at colony periphery. The green arrow indicates an intercellular gap. (c) A GM25256 hiPSC colony after 24 h of RA-induced differentiation. (d) A GM25256 hiPSC colony after 48 h of RA-induced differentiation. The red arrow indicates a jagged colony boundary. ( e ) A GM25256 hiPSC colony after 96 h of RA-induced differentiation. The red arrow indicates an intercellular gap near the colony periphery. ( f ) A <t>H9</t> hESC colony both untreated and after 24 h of RA-induced differentiation. P undiff was measured by DeepHOPE. (g) Ratio of H9 hESC colonies with average P undiff over 0.5. (h) A KOLF2.1J hiPSC colony both untreated and after 24 h of RA-induced differentiation. P undiff was measured by DeepHOPE. (i) Ratio of KOLF2.1J hiPSC colonies with average P undiff over 0.5. Scale bar (B to H) = 50 μm.
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(a) Transcriptomic analysis of the time-course differentiation process. Loss of pluripotency markers is visible over time. (b) A GM25256 hiPSC colony after 12 h of RA-induced differentiation. The red arrow indicates filopodium-like membrane protrusion at colony periphery. The green arrow indicates an intercellular gap. (c) A GM25256 hiPSC colony after 24 h of RA-induced differentiation. (d) A GM25256 hiPSC colony after 48 h of RA-induced differentiation. The red arrow indicates a jagged colony boundary. ( e ) A GM25256 hiPSC colony after 96 h of RA-induced differentiation. The red arrow indicates an intercellular gap near the colony periphery. ( f ) A <t>H9</t> hESC colony both untreated and after 24 h of RA-induced differentiation. P undiff was measured by DeepHOPE. (g) Ratio of H9 hESC colonies with average P undiff over 0.5. (h) A KOLF2.1J hiPSC colony both untreated and after 24 h of RA-induced differentiation. P undiff was measured by DeepHOPE. (i) Ratio of KOLF2.1J hiPSC colonies with average P undiff over 0.5. Scale bar (B to H) = 50 μm.
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(a) Transcriptomic analysis of the time-course differentiation process. Loss of pluripotency markers is visible over time. (b) A GM25256 hiPSC colony after 12 h of RA-induced differentiation. The red arrow indicates filopodium-like membrane protrusion at colony periphery. The green arrow indicates an intercellular gap. (c) A GM25256 hiPSC colony after 24 h of RA-induced differentiation. (d) A GM25256 hiPSC colony after 48 h of RA-induced differentiation. The red arrow indicates a jagged colony boundary. ( e ) A GM25256 hiPSC colony after 96 h of RA-induced differentiation. The red arrow indicates an intercellular gap near the colony periphery. ( f ) A <t>H9</t> hESC colony both untreated and after 24 h of RA-induced differentiation. P undiff was measured by DeepHOPE. (g) Ratio of H9 hESC colonies with average P undiff over 0.5. (h) A KOLF2.1J hiPSC colony both untreated and after 24 h of RA-induced differentiation. P undiff was measured by DeepHOPE. (i) Ratio of KOLF2.1J hiPSC colonies with average P undiff over 0.5. Scale bar (B to H) = 50 μm.
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Pluripotency and differentiating capacity <t>of</t> <t>embryonic</t> stem cells <t>(ESCs).</t> ESCs are created from the inner cell mass (ICM) of the blastocyst stage. They can develop into all three germ layers: the ectoderm (which later gives rise to the brain, skin, and eyes), the endoderm (which gives rise to the lungs, liver, and gut), and the mesoderm (which gives rise to the bones, blood, and muscles). ESCs undergo in vitro differentiation using specific protocols, forming embryoid bodies that mimic early-stage embryogenesis. They may also be implanted in vivo, where they can assist in tissue regeneration. Still, they may, in rare cases, develop into teratomas: complex tumors that contain tissues from all three germ layers. ESCs may also assist in repairing organs, although they may be unregulated and pose risks such as teratocarcinoma.
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Pluripotency and differentiating capacity <t>of</t> <t>embryonic</t> stem cells <t>(ESCs).</t> ESCs are created from the inner cell mass (ICM) of the blastocyst stage. They can develop into all three germ layers: the ectoderm (which later gives rise to the brain, skin, and eyes), the endoderm (which gives rise to the lungs, liver, and gut), and the mesoderm (which gives rise to the bones, blood, and muscles). ESCs undergo in vitro differentiation using specific protocols, forming embryoid bodies that mimic early-stage embryogenesis. They may also be implanted in vivo, where they can assist in tissue regeneration. Still, they may, in rare cases, develop into teratomas: complex tumors that contain tissues from all three germ layers. ESCs may also assist in repairing organs, although they may be unregulated and pose risks such as teratocarcinoma.
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Pluripotency and differentiating capacity <t>of</t> <t>embryonic</t> stem cells <t>(ESCs).</t> ESCs are created from the inner cell mass (ICM) of the blastocyst stage. They can develop into all three germ layers: the ectoderm (which later gives rise to the brain, skin, and eyes), the endoderm (which gives rise to the lungs, liver, and gut), and the mesoderm (which gives rise to the bones, blood, and muscles). ESCs undergo in vitro differentiation using specific protocols, forming embryoid bodies that mimic early-stage embryogenesis. They may also be implanted in vivo, where they can assist in tissue regeneration. Still, they may, in rare cases, develop into teratomas: complex tumors that contain tissues from all three germ layers. ESCs may also assist in repairing organs, although they may be unregulated and pose risks such as teratocarcinoma.
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Pluripotency and differentiating capacity <t>of</t> <t>embryonic</t> stem cells <t>(ESCs).</t> ESCs are created from the inner cell mass (ICM) of the blastocyst stage. They can develop into all three germ layers: the ectoderm (which later gives rise to the brain, skin, and eyes), the endoderm (which gives rise to the lungs, liver, and gut), and the mesoderm (which gives rise to the bones, blood, and muscles). ESCs undergo in vitro differentiation using specific protocols, forming embryoid bodies that mimic early-stage embryogenesis. They may also be implanted in vivo, where they can assist in tissue regeneration. Still, they may, in rare cases, develop into teratomas: complex tumors that contain tissues from all three germ layers. ESCs may also assist in repairing organs, although they may be unregulated and pose risks such as teratocarcinoma.
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(a) Transcriptomic analysis of the time-course differentiation process. Loss of pluripotency markers is visible over time. (b) A GM25256 hiPSC colony after 12 h of RA-induced differentiation. The red arrow indicates filopodium-like membrane protrusion at colony periphery. The green arrow indicates an intercellular gap. (c) A GM25256 hiPSC colony after 24 h of RA-induced differentiation. (d) A GM25256 hiPSC colony after 48 h of RA-induced differentiation. The red arrow indicates a jagged colony boundary. ( e ) A GM25256 hiPSC colony after 96 h of RA-induced differentiation. The red arrow indicates an intercellular gap near the colony periphery. ( f ) A H9 hESC colony both untreated and after 24 h of RA-induced differentiation. P undiff was measured by DeepHOPE. (g) Ratio of H9 hESC colonies with average P undiff over 0.5. (h) A KOLF2.1J hiPSC colony both untreated and after 24 h of RA-induced differentiation. P undiff was measured by DeepHOPE. (i) Ratio of KOLF2.1J hiPSC colonies with average P undiff over 0.5. Scale bar (B to H) = 50 μm.

Journal: bioRxiv

Article Title: Deep Learning-Guided Holotomography Reveals Early Structural Remodelling During Pluripotency Exit

doi: 10.64898/2026.04.23.720508

Figure Lengend Snippet: (a) Transcriptomic analysis of the time-course differentiation process. Loss of pluripotency markers is visible over time. (b) A GM25256 hiPSC colony after 12 h of RA-induced differentiation. The red arrow indicates filopodium-like membrane protrusion at colony periphery. The green arrow indicates an intercellular gap. (c) A GM25256 hiPSC colony after 24 h of RA-induced differentiation. (d) A GM25256 hiPSC colony after 48 h of RA-induced differentiation. The red arrow indicates a jagged colony boundary. ( e ) A GM25256 hiPSC colony after 96 h of RA-induced differentiation. The red arrow indicates an intercellular gap near the colony periphery. ( f ) A H9 hESC colony both untreated and after 24 h of RA-induced differentiation. P undiff was measured by DeepHOPE. (g) Ratio of H9 hESC colonies with average P undiff over 0.5. (h) A KOLF2.1J hiPSC colony both untreated and after 24 h of RA-induced differentiation. P undiff was measured by DeepHOPE. (i) Ratio of KOLF2.1J hiPSC colonies with average P undiff over 0.5. Scale bar (B to H) = 50 μm.

Article Snippet: The human embryonic stem cell line H9 (WA09, WiCell) and human iPSCs lines GM25256 (Coriell Institute) and KOLF2.1J (The Jackson Laboratory) were used to generate the base model for this study.

Techniques: Membrane

Pluripotency and differentiating capacity of embryonic stem cells (ESCs). ESCs are created from the inner cell mass (ICM) of the blastocyst stage. They can develop into all three germ layers: the ectoderm (which later gives rise to the brain, skin, and eyes), the endoderm (which gives rise to the lungs, liver, and gut), and the mesoderm (which gives rise to the bones, blood, and muscles). ESCs undergo in vitro differentiation using specific protocols, forming embryoid bodies that mimic early-stage embryogenesis. They may also be implanted in vivo, where they can assist in tissue regeneration. Still, they may, in rare cases, develop into teratomas: complex tumors that contain tissues from all three germ layers. ESCs may also assist in repairing organs, although they may be unregulated and pose risks such as teratocarcinoma.

Journal: Regenerative Therapy

Article Title: Engineering cardiac regeneration using stem cells: Cellular sources, differentiation signatures, targeted delivery, and functional recovery

doi: 10.1016/j.reth.2026.101120

Figure Lengend Snippet: Pluripotency and differentiating capacity of embryonic stem cells (ESCs). ESCs are created from the inner cell mass (ICM) of the blastocyst stage. They can develop into all three germ layers: the ectoderm (which later gives rise to the brain, skin, and eyes), the endoderm (which gives rise to the lungs, liver, and gut), and the mesoderm (which gives rise to the bones, blood, and muscles). ESCs undergo in vitro differentiation using specific protocols, forming embryoid bodies that mimic early-stage embryogenesis. They may also be implanted in vivo, where they can assist in tissue regeneration. Still, they may, in rare cases, develop into teratomas: complex tumors that contain tissues from all three germ layers. ESCs may also assist in repairing organs, although they may be unregulated and pose risks such as teratocarcinoma.

Article Snippet: Embryonic Stem Cells (ESCs) , - Regeneration of damaged myocardium - replacement of cardiomyocytes , Differentiation into functional cardiomyocytes , Geron Corporation studies (preclinical models) [ ] .

Techniques: Muscles, In Vitro, In Vivo